GrowthCurve 2.0

Version 2.0

Analysis setup

Analysis context

Project

Analysis mode

Quick start

Run the selected analysis mode with safe defaults and its built-in sample.

Data source

Input source

Built-in sample dataset

Existing project folder (server-managed)

Upload files (private temporary sandbox)

A built-in sample dataset is available and can be run immediately.

Upload file

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Upload 2nd file

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File format (one of: Plate, Column, Row)

Separator:

Comma

Tab

Semicolon

Space

Time intervals (either time intervals in seconds or '0' if time stamps are first column in 'Column' or 'Row' file.)

Data processing

Smooth data

Smoothing window (larger = stronger)

Remove negative growth

Background subtraction

Time points to average for background

Data analysis options ?

Growth cutoff

Enter min OD for growth curve analysis

Cutoff is inclusive, so wells reaching exactly the threshold are kept (>=).

Growth-phase method

Robust phase fit (recommended)

Legacy maximum-slope method

Robust mode requires sustained log-phase growth and reports fit confidence; legacy mode preserves historical calculations.

Control wells:

Example: A1,C1,E1,G1

Plot wells

Wells to plot:

Colours for sample groups:

Names of sample groups:

Average sample groups

Plot average of 2 or more wells with standard deviation or 95% confidence intervals

Error bars

Standard deviation

95% CI

Plot average of control wells

Colour of control:

Name of control:

Min and max OD values (format: '0;1.5'):

Time range to plot (format: '0;24'):

Add grid lines

Add frame around plot

Plate plot options

OD range for plate plot:

Specific growth-rate range (log2 OD/hour):

Add control data to wells

Median

Mean

Curve diagnostics

Well to inspect

Inspect fit support, uncertainty, derivatives, residuals, and metadata-defined replicates.

Choose OD value(s) to assess time shift (comma-separated if more than one):

Define time shift breaks

Break definitions:

Colour schema

Rescue only

Rescue and delay (blue=rescue, red=delay)

Rescue and delay (red=rescue, blue=delay)

Area under curve parameters

Define time range

Time range for area under curve analysis (hours):

Definition of controls

Control set positions:

Colours for controls:

Names for controls:

Checkerboard analysis

Choose time points for BLISS calculation (comma-separated if more than one):

Plate layout

Whole plate (8x12)

Mini checkerboards (4x4)

Two checkerboards (6x6)

Analysis status

Download pdf-file

Download Data Download Error bar data

Download pdf-file Download processed data

Lag Exponential Deceleration Stationary Decline Bands are hidden when robust-fit confidence is low.

GrowthCurve plate and screen growth analysis

Parameter plots

Specific growth rate and detected exponential phase

Table: Growth curve parameters

Selected-well metrics

Plate quality and replicate context

Data table(s) - OD time & time shift

Log-phase parameters (hours)